Ziehl-Neelsen stain for Acid-fast Bacteria (AFB) – 1st sample

. Tuberculosis is an infectious disease caused by the bacterium Mycobacterium tuberculosis.
. The disease can affect all parts of the body, with pulmonary tuberculosis being the most common form (accounting for 80–85% of total cases) and serving as the primary source of transmission to others.
. Direct sputum smear for AFB: all people with suspected pulmonary tuberculosis symptoms must have their sputum tested.
. To facilitate the patient's diagnosis on the day of the medical examination, testing 2 sputum samples on-site should be applied, instead of testing 3 sputum samples as before. The patient should be carefully instructed on how to collect the sputum sample correctly, the time of sample 1 and sample 2 must be at least 2 hours apart.
Source: Guidelines for diagnosis, treatment, and prevention of tuberculosis No. 1314/QD-BYT 2020.

  • Infectious/ Contagious diseases

Patient Instructions:
. Two on-the-spot sputum samples are taken, at least 2 hours apart.
. To ensure the collection of sputum, not saliva or nasal secretions, follow the following steps:
1. Ensure that your mouth is clean
2. Inhale deeply, hold the air for 5 seconds, then exhale strongly twice.
3. Take a third deep breath and, on the exhalation, cough deeply from within your lungs to bring the sputum into the cup. Ensure the sputum goes directly to the bottom of the cup.
4. Tighten the lid securely on the cup.
Sample Type:
Sputum
Result Time:

After 3 hours
After cutoff: 8:00 AM next day

Storage & Transportation:
-
Cutoff Times:

7:00 PM Monday – Saturday
7:00 PM Sunday

30 minutes required for samples arrived at Cao Thang till receive by Laboratory

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