Free Triiodothyronine (Free T3) test measures the amount of T3 hormone that is not attached to transport proteins in the blood. T3 is one of two major hormones made by the thyroid, a small gland located near the throat. The Thyroid helps regulate energy use, weight, body temperature, muscle strength, and the nervous system. This test helps diagnose, assess and monitor thyroid disorders, such as an overactive or underactive thyroid gland and aids diagnoses of pituitary gland disorders.